goat anti neun  (Novus Biologicals)

Journal: CNS Neuroscience & Therapeutics

Article Title: METTL14 ‐mediated upregulation of lncRNA HOTAIR represses PP1α expression by promoting H3K4me1 demethylation in oxycodone‐treated mice

doi: 10.1111/cns.14830

Figure Lengend Snippet: Oxycodone downregulated striatal PP1α underlying m6A methylation. (A) Oxycodone (1.5, 3.0, and 6.0 mg/kg) was injected to all test animals. (B–D) Western blotting shows a significant increase in METTL14 expression and a decrease in PP1α expression. (E) Oxycodone markedly increased the movement of mice. * p < 0.05, ** p < 0.01, *** p < 0.001, vs. baseline (day 1), two‐way ANOVA. (F–H) Western blotting shows the expressions of METTL14 and PP1α in striatal neurons. * p < 0.05, ** p < 0.01, *** p < 0.001, vs. SAL group or day 1, one‐way ANOVA. Representative images of double immunofluorescence staining (I) of METTL14 or PP1α (red) and NeuN (green). METTL14 (J), m6A (L), and HOTAIR (M) levels were upregulated, while PP1α level (K) was downregulated in oxycodone‐treated mice. Scale bar (E) = 20 μm. * p < 0.05, ** p < 0.01, *** p < 0.001, vs. SAL group or day 1, one‐way ANOVA.

Article Snippet: Subsequently, after washing with TBS three times, blocking using 5% goat serum (Cat# C0265; Beyotime), and incubation overnight with primary antibodies against PP1α (Cat# BM4356; Boster), METTL14 (Cat# Ab308576; Abcam, UK), and NeuN (Cat# Ab104224) at 4°C, these slices were incubated with secondary antibodies (DyLight 488 or 594 Conjugated AffiniPure Goat, Anti‐rabbit, or Mouse lgG(H + L); Cat# BA1126, BA1127, BA1141, BA1142; Boster, China) for 1 h at room temperature.

Techniques: Methylation, Injection, Western Blot, Expressing, Double Immunofluorescence Staining

Journal: Heliyon

Article Title: Sub-region expression of brain-derived neurotrophic factor in the dorsal hippocampus and amygdala is Affected by mild traumatic brain injury and stress in male rats

doi: 10.1016/j.heliyon.2023.e23339

Figure Lengend Snippet: Effects of mTBI and stress on BDNF expression in the Dorsal Hippocampus: Representative epifluorescent image (20x) of labeling of BDNF (red) and NeuN (green) in the Cornu ammonis sub-region 1 (CA1) of the dorsal hippocampus (A) shows that BDNF in the dorsal CA1 was lower in rats receiving drug treatment than those receiving vehicle injection prior to mTBI, and no differences between mifepristone and spironolactone treatments were observed (D). Representative image (B) of the cornu ammonis sub-region 3 (CA3) shows BDNF did not differ between groups receiving mTBI concurrent with social defeat stress and controls or between groups treated with vehicle, mifepristone, or spironolactone (E). Representative image (C) of the dentate gyrus (DG) showed that BDNF intensity was lower in rats receiving drug treatment than those receiving vehicle injection prior to mTBI concurrent with social defeat stress, and there were no differences between mifepristone and spironolactone treatments (F). Dashed line circles in A, B, and C represent adjacent regions quantified for background noise calculation. * = Significant difference (P < 0.05) compared to vehicle group, n = 5–6/group. Abbreviations: mild traumatic brain injury (mTBI), brain-derived neurotrophic factor (BDNF), neuronal nuclei (NeuN), cornu ammonis sub-region 1 (CA1), cornu ammonis sub-region 3 (CA3), dentate gyrus (DG). Arrows indicate BDNF labeling. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Next, sections were incubated at room temperature for 2 h in 1:200 Alexa Fluor goat anti-mouse NeuN secondary antibody (#115-545-146, Jackson ImmunoResearch, West Grove, PA, USA) and 1:400 Cy3 goat anti-rabbit BDNF secondary antibody (#111-165-144, Jackson ImmunoResearch, West Grove, PA, USA) [ ].

Techniques: Expressing, Labeling, Injection, Derivative Assay

Journal: Heliyon

Article Title: Sub-region expression of brain-derived neurotrophic factor in the dorsal hippocampus and amygdala is Affected by mild traumatic brain injury and stress in male rats

doi: 10.1016/j.heliyon.2023.e23339

Figure Lengend Snippet: Effects of mTBI and stress on BDNF expression in the Amygdala: Representative epifluorescent image (20x) of labeling of BDNF (red) and NeuN (green) in the medial nucleus (MeA) of the amygdala (A) shows that BDNF in the MeA was greater in rats receiving mTBI with social defeat stress than controls among the treatment groups (D). Representative image (B) of the basolateral amygdala (BLA) shows that BDNF did not differ between groups receiving mTBI with social defeat stress and controls or between groups treated with vehicle, mifepristone, or spironolactone (E). Representative image (C) of the central nucleus of the amygdala (CeA) showed that BDNF intensity was lower in rats receiving drug treatment than those receiving vehicle injection prior to mTBI with social defeat stress, and there were no differences between mifepristone and spironolactone treatments (F). Dashed line circles in A, B, and C represent adjacent regions quantified for background noise calculation. # = Significant difference (P < 0.05) compared to sham group, * = Significant difference (P < 0.05) compared to vehicle group, n = 5–6/group. Abbreviations: mild traumatic brain injury (mTBI), brain-derived neurotrophic factor (BDNF), neuronal nuclei (NeuN), medial nucleus of the amygdala (MeA), basolateral amygdala (BLA), central nucleus of the amygdala (CeA). Arrows indicate BDNF labeling. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Next, sections were incubated at room temperature for 2 h in 1:200 Alexa Fluor goat anti-mouse NeuN secondary antibody (#115-545-146, Jackson ImmunoResearch, West Grove, PA, USA) and 1:400 Cy3 goat anti-rabbit BDNF secondary antibody (#111-165-144, Jackson ImmunoResearch, West Grove, PA, USA) [ ].

Techniques: Expressing, Labeling, Injection, Derivative Assay